FastQCFastQC Report
Sun 11 Sep 2022
EGAF00000710455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000710455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3332679
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAACCTAATCAAGCTTGAGCTCCACCGAAACCAGCACGGAATTTCTA36708911.01483221156313No Hit
ACCTAACCTAATCAAGCTTGTAAAGAATTTTGTGAGCTGAAGTATTTTAA1767175.302550890739853No Hit
ACCTAACCTAATCAAGCTTTTATCACAATGAAAGAAATACTCAAAGCATG1228673.686733705826454No Hit
ACCTAACCTAATCAAGCTTTCCTAGGGTGCTACACAGATATATAAAGCTG1033433.1008987064160696No Hit
ACCTAACCTAATCAAGCTTCCCGTTTTTCTAGGTGAGTGTCCCAAAGTTA959052.8777148954339737No Hit
ACCTAACCTAATCAAGCTTAACTCCCTGACAAAACTAGGAATTCCCTGAA434821.3047161157735263No Hit
ACCTAACCTAATCAAGCTTTTCTGCTTCTTAGGGGGCTTTAATCTAAAGA268630.8060482272670125No Hit
ACCTAACCTAATCAAGCTTTGGAGGACAAGATGTGCTGATCCCTATTTTA249490.7486169535079736No Hit
ACCTAACCTAATCAAGCTTTGGAGTCATATCTGGGCCTGTATCTGTCTCT169870.5097100560840092No Hit
ACCTAACCTAATCAAGCTTCTTGCAGCACCTTTTTTTCACTGTCGCATCA169780.5094400030726032No Hit
ACCTAACCTAATCAAGCTTAATCAGGCAGGACATTAGAGTCCAGGGGGGC165800.4974976587904205No Hit
ACCTAACCTAATCAAGCTTGCAAAAGGAGATCCCTATTTTACTTTTACGT164700.49419701087323437No Hit
ACCTAACCTAATCAAGCTTATACATAGTAGAGCAAGGAATTGAACCTAAA155580.46683163905074565No Hit
ACCTAACCTAATCAAGCTTATTTTGAGGGTAGCAACATGGCGTTCCCAAC154480.46353099113355956No Hit
ACCTAACCTAATCAAGCTTCTCCACAGTGAAGGAAACAATAAACAGACTA148550.4457374982709106No Hit
ACCTAACCTAATCAAGCTTGTTTTCAGCCAGTGTAAGCGTGGTGTTCTTG140590.4218528097065454No Hit
ACCTAACCTAATCAAGCTTTGAACCTAAGTTGAACCTTCCAACCCCGGCA132030.39616776773280593No Hit
ACCTAACCTAATCAAGCTTGAGTCTGGGGAGAAGGATGGTGGGAAAGAAA126270.378884375002813No Hit
ACCTAACCTAATCAAGCTTCCAACTAATGAGAATGAATCTCATCAATGTC124900.3747735680514085No Hit
ACCTAACCTAATCAAGCTTGATGCAGTGGTTCACACCTGTAATCCCATCA122440.36739211907297403No Hit
ACCTAACCTAATCAAGCTTTGAGGTCTGCACATCAGATAGCATTACTCCC119410.358300334355634No Hit
ACCTAACCTAATCAAGCTTTCATGCTATGTTCAGTAAGGCAGAATCAAAT114660.3440475365314211No Hit
ACCTAACCTAATCAAGCTTTTGCTTTCACTCCAATTAAAACAACAATGGT110750.3323152334803322No Hit
ACCTAACCTAATCAAGCTTGATCCCTATTTTACTTTTACGTGCTTAATCC110110.33039485651033296No Hit
ACCTAACCTAATCAAGCTTCACAGCTGCTGATGGACACAGACACTGGGAC105250.31581199389440145No Hit
ACCTAACCTAATCAAGCTTAGCAACATATTCTGAGTCATCATGGGAGAAT101570.304769826316906No Hit
ACCTAACCTAATCAAGCTTTACAGTGGCTCCATTTCACTCAGGCTCACCA97510.2925874349134735No Hit
ACCTAACCTAATCAAGCTTCTCGCACTAAACAGCCAAGTTGTGAATGCAA97190.29162724642847393No Hit
ACCTAACCTAATCAAGCTTAGTTTGGCTGGATATGAAATTCTGGGTTGAA96000.28805654549988163No Hit
ACCTAACCTAATCAAGCTTTAGAAAACTCTTTTTTCTTATCAGAATCTAG94380.2831955912945711No Hit
ACCTAACCTAATCAAGCTTAGTCATCTGACTTAGAAAGGACAGAAACTAT93220.27971490803644755No Hit
ACCTAACCTAATCAAGCTTTGATTTTTGCAGCTCCCCTGAAGATTTGGAA90100.2703530703077014No Hit
ACCTAACCTAATCAAGCTTCAATTATAAAATGAATGACAGATGCACAAGA89050.26720245184129643No Hit
ACCTAACCTAATCAAGCTTCAACTACGAATAAATAGCACCCATCTGCTTG85860.25763057288145663No Hit
ACCTAACCTAATCAAGCTTCCAAATCAGGTGACTTTCAGTTAATAAAAGA84140.25246955977458374No Hit
ACCTAACCTAATCAAGCTTCCTAAATCATTAAAAGGATATCCTTTTAAAG82110.2463783640728675No Hit
ACCTAACCTAATCAAGCTTATCCACCATGATCCCTATTTTACTTTTACGT81970.24595828161068015No Hit
ACCTAACCTAATCAAGCTTCCAGCACCAGACAGGGAAGAAGGAGTTCTGA78320.23500613170365342No Hit
ACCTAACCTAATCAAGCTTTATACCAGATGAAGTAAATAGAAAAGAAGAA76960.23092533064240509No Hit
ACCTAACCTAATCAAGCTTACCTCATTTTGGTTTTGTCAATAAAGGGTCA75850.2275946768350627No Hit
ACCTAACCTAATCAAGCTTGATTATGGGTGATGCTAGGCCCAATTTTCAC75840.22756467094490646No Hit
ACCTAACCTAATCAAGCTTCAATATCTGAGGGCAGGAGAAGGTAGATATC75030.22513419384225125No Hit
ACCTAACCTAATCAAGCTTTTCTTTCAAGGGTGAATAATATTAATACTTG71840.21556231488241143No Hit
ACCTAACCTAATCAAGCTTGTTTTGAAGTCTAAAGGCCCAATATGTCTCA70870.21265174353725635No Hit
ACCTAACCTAATCAAGCTTTTTTATTTAAAAAAACCCAATAACTCAGTTT70810.21247170819631891No Hit
ACCTAACCTAATCAAGCTTGTCCACTTAATTACAGAGAAGGAAAAACTAA68300.2049402297671033No Hit
ACCTAACCTAATCAAGCTTTCTTAGCACAGAAGGAGTAAACTGCAAATGA67620.20289982923647915No Hit
ACCTAACCTAATCAAGCTTCATAAGTGAAGGAGAAATAAAATACTTTACA67340.20205966431210445No Hit
ACCTAACCTAATCAAGCTTCCTCTTGGGAGAATTAGGGAAGTTTCAAGGC62700.18813693127961018No Hit
ACCTAACCTAATCAAGCTTTCTATTTTGTTAAATCTTTTTATGCTTGGCT62530.18762683114695416No Hit
ACCTAACCTAATCAAGCTTAATGCAGAAAATATACCCAAGTCAGGAGGTA59270.17784491095602067No Hit
ACCTAACCTAATCAAGCTTTAGTGTGCTCAGAATGACCAGAGAACCTTGT59100.17733481082336464No Hit
ACCTAACCTAATCAAGCTTGCCTGGTTCTTAGGCCTTCAGATCCCTATTT58950.17688472247102105No Hit
ACCTAACCTAATCAAGCTTGAGAAAGTTTCTGCATTCCCTTGTTAATTTC58330.17502435728133434No Hit
ACCTAACCTAATCAAGCTTCTCTGTTCCCTGATGCAGTGTGGATATTTTT56660.17001337362524263No Hit
ACCTAACCTAATCAAGCTTTTTTTATTTAAAAAAACCCAATAACTCAGTT54770.1643422603857137No Hit
ACCTAACCTAATCAAGCTTCAGAACACCTTGGAATACAGTTTAGCTTCCT54570.16374214258258896No Hit
ACCTAACCTAATCAAGCTTAGTCCTGAGACCCGGGGAGATTAAAAAAAAA53870.16164173027165232No Hit
ACCTAACCTAATCAAGCTTAAAGTGTCAATTTTTATACTTATCTGGTTTG51370.1541402577325929No Hit
ACCTAACCTAATCAAGCTTATCCACCACGATCCCTATTTTACTTTTACGT50710.15215986898228123No Hit
ACCTAACCTAATCAAGCTTATTTGAGCCTGTTGGGGTGAGGTGGGTGCAA49910.1497593977697822No Hit
ACCTAACCTAATCAAGCTTGGGCCTGTGATGTTCATCTCAGCCCCATGTG49080.1472689088868145No Hit
ACCTAACCTAATCAAGCTTCGTCTTCATGAAACTTATATTTTAGAAGGGG47630.14291805481416003No Hit
ACCTAACCTAATCAAGCTTACCCAGTGTGTTAGGCATAATACTAGCCCTC47350.14207788988978537No Hit
ACCTAACCTAATCAAGCTTCATAAGTGAAGGAGAAATAAAATCCTTTACA47240.14174782509806674No Hit
ACCTAACCTAATCAAGCTTAGGTTCAGGCAATCTTCAGAATCAAGAAGAC46380.1391673185446303No Hit
ACCTAACCTAATCAAGCTTTAAGCCAAGCAAAAGAGTTTGGATTTTTTCC46290.13889726553322415No Hit
ACCTAACCTAATCAAGCTTCATAAAAGTATAAGCAATTCCAGATTCAAAA45530.1366168178813501No Hit
ACCTAACCTAATCAAGCTTCCCAGTCACTTTGTTTACGTACTCAAGCCTC44950.13487647625228832No Hit
ACCTAACCTAATCAAGCTTTTACATAGCTCAGAATAATTTTTATCATTAC44660.13400630543775743No Hit
ACCTAACCTAATCAAGCTTAGTTTGTCTGGATATGAAATTCTGGGTTGAA41990.12599473276604198No Hit
ACCTAACCTAATCAAGCTTACTGTCCCATGAATTATCATAATATGACAAA41880.12566466797432335No Hit
ACCTAACCTAATCAAGCTTTTTTCTATTAAATTTTTTTTATTACTTTTAA41790.12539461496291723No Hit
ACCTAACCTAATCAAGCTTTCAAAAGTAGGAAGCTGGTCAATGAGAATTT40780.12236402005713723No Hit
ACCTAACCTAATCAAGCTTTATACCAGACGAAGTAAATAGAAAAGAAGAA40290.12089373143948157No Hit
ACCTAACCTAATCAAGCTTCTGACTTAAGTGCCCTGAAAAGGCTTTATGG40170.12053366075760671No Hit
ACCTAACCTAATCAAGCTTCTTTTAAGAAATCAGACTGTTTATACCCCAA40010.12005356651510692No Hit
ACCTAACCTAATCAAGCTTGCATGTCTGCCTTCTTCCTCATAGAAACACT38650.11597276545385861No Hit
ACCTAACCTAATCAAGCTTTTCTGTTATCCTGACTAGAACTTACCATCTT38080.11426242971495305No Hit
ACCTAACCTAATCAAGCTTCTGGACAGCAAAAGAAATAATCATCAGAGTA37930.11381234136260948No Hit
ACCTAACCTAATCAAGCTTTATGAAAAAGAGGCCAGAGGAGGGAGACTGG37390.11219202329417265No Hit
ACCTAACCTAATCAAGCTTCACGGTGCAGTACATTTCCATATGGACAGGG36720.11018162865370472No Hit
ACCTAACCTAATCAAGCTTAGCAAGAATTTTAAATTACTGAGTAAATGAA36480.10946148728995503No Hit
ACCTAACCTAATCAAGCTTCAAATGGGAAGGATCCCTATTTTACTTTTAC35680.107061016077456No Hit
ACCTAACCTAATCAAGCTTTACAATACCCTGATATATAGGAATTATACTA35410.10625085704323758No Hit
ACCTAACCTAATCAAGCTTATCCACCAGGATCCCTATTTTACTTTTACGT34950.10487058609605066No Hit
ACCTAACCTAATCAAGCTTGCCAACCTCTCCTCTAATCTAGTCTGCCTTG34940.1048405802058944No Hit
ACCTAACCTAATCAAGCTTCTACCACCTCTTCTGGAATGGTTCTAGCTAG34910.10475056253542571No Hit
ACCTAACCTAATCAAGCTTTCTAGTCTTTCTCAAGGAAACCTGTCCAAAG34850.10457052719448827No Hit
ACCTAACCTAATCAAGCTTTTTGATGTGCTGCTGGATTCGGTTTGCCAGT34730.10421045651261343No Hit
ACCTAACCTAATCAAGCTTGAACAAAGCACAGTGGATATTGAAAGAAAGA34650.10397040939136354No Hit
ACCTAACCTAATCAAGCTTTAAACCTTTTGGAATGTAAAGGCATATTTGT34550.10367035048980115No Hit
ACCTAACCTAATCAAGCTTGGATTATCCAGGAGGGAAACCTGACAATATA34470.10343030336855123No Hit
ACCTAACCTAATCAAGCTTTTCTGCCAGACTACTCAGAAGTAAAGTGATT34210.10265015022448906No Hit
ACCTAACCTAATCAAGCTTTTTAGAAAGAAAAACCATACAGCAGTATGGT34160.10250012077370789No Hit
ACCTAACCTAATCAAGCTTACTAAATTTTTCAGTTAATGATGGTGGCATT33410.10024967901199006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)