FastQCFastQC Report
Sat 1 Apr 2023
EGAF00000718615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000718615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34854501
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC1488630.4270983538108894No Hit
GGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAT1218370.3495588704598009No Hit
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAA1104830.31698345071702505No Hit
CCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC1069890.3069589204562131No Hit
TGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCAT836060.23987145878232485No Hit
CTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTG814130.23357958847266239No Hit
GGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGG771550.2213630888016443No Hit
GTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGC625080.17933982184969455No Hit
CCGAAAGTGAACGGTGAACTACCACACTGCTGACAGTAATACACTGCAAAA618500.17745197384980493No Hit
GTTCACCGTTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTG587820.16864966737007653No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCA582400.16709463147958997No Hit
CCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGG580900.16666427099329292No Hit
TGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATA580250.1664777814492309No Hit
GCTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCTG552710.15857636292081762No Hit
CACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGG538880.15460843923715906No Hit
CTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGT533070.15294150962023528No Hit
GGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCT531850.15259148309138038No Hit
GAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGG518660.14880717988187522No Hit
CTGGGACAGACTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATTTTG511430.14673284233792358No Hit
CTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCAGT505650.14507451993072573No Hit
CTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATTTTGCAGTGTATTA497560.14275344237463045No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATG481760.1382203119189685No Hit
CTGGGAGCCTGGCCAGGTTTGTGCTGGTACCAGACTAAGTAGTCGCTAACA473210.1357672571470755No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC465410.133529382618331TruSeq Adapter, Index 3 (100% over 51bp)
CTGGCATCCCAGACAGGTTCACTGGCAGTGGGTCTGGGACAGACTTCACTC442930.12707971346369296No Hit
GTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAACGGTGAACTA438780.12588904945160453No Hit
GAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCC438390.12577715572516732No Hit
GTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGG434680.12471273078905934No Hit
CTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCT433960.12450615775563678No Hit
TGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAG419960.12048945988353124No Hit
CGACTACTTAGTCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCTCCT417320.1197320254276485No Hit
ATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCC405270.1162747961877291No Hit
CCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCACCGG403380.11573254197499484No Hit
CTAACACTCTCACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGAC393080.11277739996908864No Hit
CTGGGATGCCAGTGGCCCTGCTGGATGCACCATAGATGAGGAGCCTGGGAG388200.11137729385366901No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCC383840.11012637937349899No Hit
CCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGG377310.10825287672315263No Hit
CTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCA376540.10803195834018682No Hit
CTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGC364710.10463784863825765No Hit
CCCAGACCCACTGCCAGTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGA362750.1040755109361629No Hit
CTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAG362050.1038746760425576No Hit
GAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGG360250.10335824345900117No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGA356400.10225365154417215No Hit
GGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC355210.1019122322250432No Hit
GAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATC352740.10120357195760742No Hit
CACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAACGGTGA351390.1008162475199401No Hit
CCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAACGGTG350390.10052934052907543No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)