FastQCFastQC Report
Sat 11 May 2024
EGAF00000718615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000718615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34854501
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTT1491080.4278012759385079No Hit
GGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATA1219840.34998062373637195No Hit
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAA1107450.3177351470330905No Hit
CCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC1070500.3071339337206406No Hit
TGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCA837150.24018418740236733No Hit
CTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCT815200.2338865789528876No Hit
GGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTG772040.221503673227168No Hit
GTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAG625900.17957508558220356No Hit
CCGAAAGTGAACGGTGAACTACCACACTGCTGACAGTAATACACTGCAAA619300.1776814994424967No Hit
GTTCACCGTTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACT588140.16874147760715322No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC582650.16716635822730613No Hit
CCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGG581660.16688232030635008No Hit
TGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAT580850.16664992564374972No Hit
GCTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCT552870.15862226803935595No Hit
CACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAG539390.15475476180250006No Hit
CTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTG533710.15312513009438866No Hit
GGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGC532110.1526660789090052No Hit
GAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGG519020.14891046639858652No Hit
CTGGGACAGACTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATTTT511850.14685334327408675No Hit
CTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCAG507070.14548192785775357No Hit
CTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATTTTGCAGTGTATT497910.1428538598214331No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT481930.1382690861074155No Hit
CTGGGAGCCTGGCCAGGTTTGTGCTGGTACCAGACTAAGTAGTCGCTAAC473800.13593653227168567No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC465980.13369291960312385TruSeq Adapter, Index 3 (100% over 50bp)
CTGGCATCCCAGACAGGTTCACTGGCAGTGGGTCTGGGACAGACTTCACT443270.12717726184058695No Hit
GTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAACGGTGAACT439140.12599233596831583No Hit
GAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC438620.12584314433306618No Hit
GTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTG435010.1248074100960447No Hit
CTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGC434240.1245864917130789No Hit
TGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAA425310.12202441228465728No Hit
CGACTACTTAGTCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCTCC417910.11990130055225866No Hit
ATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCC405410.11631496316645015No Hit
CCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCACCG403510.11576983988380726No Hit
CTAACACTCTCACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGA393450.11288355555570857No Hit
CTGGGATGCCAGTGGCCCTGCTGGATGCACCATAGATGAGGAGCCTGGGA388550.11147771130047164No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC384100.1102009751911238No Hit
CCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAG377470.10829878184169099No Hit
CTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTC377050.10817828090552782No Hit
CTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGG365080.10474400422487759No Hit
CCCAGACCCACTGCCAGTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGG363280.10422757164132115No Hit
CTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCA362180.10391197395137No Hit
GAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAG360330.10338119601827035No Hit
GGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGG359170.10304838390886734No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGG356930.10240571224933044No Hit
GAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAT353250.10134989452294842No Hit
CACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAACGGTG351570.10086789077829576No Hit
CCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAACGGT350630.10059819820688294No Hit

[OK]Adapter Content

Adapter graph