FastQCFastQC Report
Sat 11 May 2024
EGAF00000718616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000718616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34386869
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC912550.2653774613792259TruSeq Adapter, Index 2 (100% over 50bp)
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAA599440.174322355431662No Hit
CTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTT535790.1558123829186077No Hit
CCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGCAAACTGG525530.1528286858568019No Hit
GGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACCTTG493340.14346755443189668No Hit
TGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTC444130.1291568592650875No Hit
CCGAGAGCCACAGGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCA434670.12640580914767202No Hit
CTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCT424390.1234162959122565No Hit
CAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTG361600.10515641886442177No Hit

[OK]Adapter Content

Adapter graph