FastQCFastQC Report
Tue 27 Sep 2022
EGAF00000718617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000718617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35373380
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAA1143030.3231328190859906No Hit
CTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC980920.27730457196909086No Hit
CTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTG799390.22598632078698727No Hit
CCCAGATCCACTGCCGCTGAATCTCGATGGGACCCCATCTTCCAAAGTGGA692510.1957715095362671No Hit
TGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC632460.1787954670998361No Hit
GGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGG602170.17023253079010262No Hit
TGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCAT559600.15819805740927217No Hit
CGGCAAGTGATGGTGACTCTGTCTCCCACAGATGCAGACAGGGAGGAAGGA556250.15725101757310156No Hit
GTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGC544890.15403956308387834No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT520770.1472208762634501No Hit
GTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGG516130.14590915541573918No Hit
CCGACTGTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGA481510.13612213478044788No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCA468090.13232832146659437No Hit
CTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGT457850.1294334892509565No Hit
CCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC437860.1237823470643744No Hit
CAAAGATGAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCA437700.12373711531100506No Hit
CTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCT436450.12338374223780707No Hit
CTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACACCTG429840.12151510542673614No Hit
TGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAG416060.11761952066780161No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC410230.11597138865440623TruSeq Adapter, Index 6 (100% over 51bp)
CAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGC408100.11536924093767686No Hit
GTGGAGTTGAGGGAGATGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCA401690.11355714381831761No Hit
GTGGAGTTGAGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGGATTCG398640.11269491351971456No Hit
CCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGG397860.112474408722039No Hit
CAGAATATTAATATGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCC394290.11146517522498556No Hit
CCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGG392780.11103830055256242No Hit
CTTGGCGTTGTCTCTGGAGATGGTGAATCGGCCCTTTACGGAGTCCGCGTA392740.11102699261422008No Hit
CGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGG389480.11010539563931973No Hit
CTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCGCTGTTCA388340.10978311939656318No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATG387940.10967004001313982No Hit
CGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCG387150.1094467082308787No Hit
GCTGTTTCTGCTTGCTGATCAGGACTGCACACAGAGAACTCACCATGGAGT380400.10753849363560959No Hit
CTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAG376770.10651229823104268No Hit
CGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAG366030.1034761167861256No Hit
GTGACATCCAACTGACCCAGTCTCCTTCCTCCCTGTCTGCATCTGTGGGAG363550.10277502460890081No Hit
CTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACA360660.10195802606366708No Hit
GCTGAATCTCGATGGGACCCCATCTTCCAAAGTGGAGGCATCAAAGATGAG359500.10163009585173936No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)