FastQCFastQC Report
Thu 5 Jan 2023
EGAF00000718618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000718618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31424457
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC1643830.5231052997988159TruSeq Adapter, Index 7 (100% over 51bp)
CTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGCACCGAGGATT1447810.46072713364625517No Hit
CCAGGCTTGACCAGGCCTCCCCCAGACTCCACCAGCTGCACCTCACACTGG592300.18848376600429406No Hit
CTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC530270.16874436366553605No Hit
CTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGCACC506530.1611897382984215No Hit
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAA484660.1542301908351193No Hit
TGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC432280.13756164505881519No Hit
CCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAA431130.1371956880591445No Hit
GGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCTAGCTTGG416840.13264827455888895No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC408200.12989882370918931TruSeq Adapter, Index 7 (100% over 50bp)
CTTATTCGTACACTTTTGGCCAGGGGACCAAGCTAGAGATCAAACGAACTG401900.12789401579794998No Hit
CCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAACTAGA398740.1268884296075506No Hit
GTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCC362410.11532737065273714No Hit
GAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTAC354660.11286113869843478No Hit
CTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTG336810.10718084961659002No Hit
CACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGG334980.1065985006518967No Hit
CGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAG333290.1060607029741198No Hit
CAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGC330920.10530651333132024No Hit
GTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACC328430.10451413687116375No Hit
CTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGAGTTCTTGGCGTTGTC317630.10107732330903921No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)