FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000718619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000718619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46940885
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAA811570.17289192566352338No Hit
GGAAACCCCAGCGCAACTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAG794730.16930443471613288No Hit
CTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC757300.16133057568045425No Hit
CCGGGATGTCAGTGGCCCTCTTGGATGCGCCATAGATGAGGAGCCTGGGAG686370.14622008085275767No Hit
CAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGCGCATCCAAGAGG601850.12821445526644842No Hit
GGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTGTGG587500.12515741874061387No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT553570.11792917836977296TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG552020.11759897581820199TruSeq Adapter, Index 16 (97% over 40bp)
CTGGGACATACTTCACTCTCACCATCAGCCGACTGGAGCCTGAAGATTTTG523310.11148277242749044No Hit
CTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTG516230.10997449238547591No Hit
CTTGGATGCGCCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTG485780.10348761000138793No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)