FastQCFastQC Report
Thu 9 May 2024
EGAF00000718619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000718619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46940885
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAA813940.17339681601657062No Hit
GGAAACCCCAGCGCAACTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATA795360.16943864607580364No Hit
CTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTT758660.16162030179021977No Hit
CCGGGATGTCAGTGGCCCTCTTGGATGCGCCATAGATGAGGAGCCTGGGA687580.14647785187688728No Hit
CAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGCGCATCCAAGAG602540.12836144866037358No Hit
GGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTGTG588080.12528097840507268No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA554540.11813582125688511TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT552960.11779922768818696TruSeq Adapter, Index 16 (97% over 40bp)
CTGGGACATACTTCACTCTCACCATCAGCCGACTGGAGCCTGAAGATTTT523720.1115701163282286No Hit
CTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCT517240.11018965662875763No Hit
CTTGGATGCGCCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCT486110.1035579111897869No Hit

[OK]Adapter Content

Adapter graph