FastQCFastQC Report
Sat 11 May 2024
EGAF00000718620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000718620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41270805
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCC879700.2131530993882964No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA759030.1839145129347489TruSeq Adapter, Index 19 (97% over 40bp)
CTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCC751580.18210936278078416No Hit
CGGGGATGAGGCCGACTACTACTGTCAGGTGTGGGACAGTAGTACTGATC661720.16033610199752585No Hit
CCGGGGATGAGGCCGACTACTACTGTCAGGTGTGGGACAGTAGTACTGAT581930.14100282269754613No Hit
CGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGA559050.13545895215758452No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT531640.1288174534031987TruSeq Adapter, Index 19 (97% over 40bp)
CCAGACTCCAACAGCTGCACGTCACACTGGACACCTTTTAAAATAGCCAC531230.1287181095692221No Hit
CGGACATATATTACTGTGCGACTTCGAACGATTTTTGGAGTGGTCCACTC518380.1256045284311755No Hit
CTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACT517470.1253840335801543No Hit
CCCGGCTGTACCAAGCCTCCCCCAGACTCCAACAGCTGCACGTCACACTG500480.12126732202097827No Hit
CTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCA490670.1188903390665629No Hit
CTGGGATTGTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGAGAA467430.11325923979432918No Hit
CTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCGCTGTTC448710.10872334571617878No Hit
GCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC446300.10813939781402374No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCA427940.10369073246814546No Hit
CCCAGCCCTGGGATTGTCAGGTGTTTTCATTTGGTGATCAGGACTGAACA425070.10299532563030937No Hit
CTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACACCT414430.10041723198759996No Hit

[OK]Adapter Content

Adapter graph