FastQCFastQC Report
Wed 14 Sep 2022
EGAF00000718620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000718620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41270805
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCT878980.21297864192375218No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT758140.1836988641244095TruSeq Adapter, Index 19 (97% over 40bp)
CTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCT751260.1820318261298756No Hit
CGGGGATGAGGCCGACTACTACTGTCAGGTGTGGGACAGTAGTACTGATCA661180.16020525889911766No Hit
CCGGGGATGAGGCCGACTACTACTGTCAGGTGTGGGACAGTAGTACTGATC581380.14086955657879704No Hit
CGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGAC558670.13536687738463063No Hit
CCAGACTCCAACAGCTGCACGTCACACTGGACACCTTTTAAAATAGCCACA530960.12865268802001803No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG530920.12864299593865447TruSeq Adapter, Index 19 (97% over 40bp)
CGGACATATATTACTGTGCGACTTCGAACGATTTTTGGAGTGGTCCACTCA518030.12551972271924425No Hit
CTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTG517090.1252919588072004No Hit
CCCGGCTGTACCAAGCCTCCCCCAGACTCCAACAGCTGCACGTCACACTGG499900.12112678684120652No Hit
CTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCAC490100.11875222690713204No Hit
CTGGGATTGTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGAGAAC466980.11315020387898903No Hit
CTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCGCTGTTCA448560.10868700041106541No Hit
GCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCC445820.1080230928376609No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCAC427250.10352354406462388No Hit
CCCAGCCCTGGGATTGTCAGGTGTTTTCATTTGGTGATCAGGACTGAACAG424770.10292263502008261No Hit
CTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACACCTG414170.10035423345873674No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)