FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000718623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000718623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45632919
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAA1173290.2571148253741997No Hit
CTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC1048100.2296806829297946No Hit
CCTGACTCGCCCGACAAGTGATGTTGACTCTGTCTCCTACAGATGCAGACA969420.21243874405667537No Hit
CCCAGATCCGCTGCCGCTGAACTTTGATGGGACCCCACTCTGCAAACTGGA904000.19810260220258974No Hit
CTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTG893660.1958366941198743No Hit
CCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC801130.1755596656001778No Hit
GGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGG746230.16352887703721078No Hit
TGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC726500.15920524391612995No Hit
CGACAAGTGATGTTGACTCTGTCTCCTACAGATGCAGACAGTGAAGATGGA696490.15262885111513466No Hit
TGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTG665340.14580263866091933No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCA656770.14392460846083505No Hit
CCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATG643520.14102100284226832No Hit
CAAAGTTCAGCGGCAGCGGATCTGGGACAGAGTTCATTCTCACCATCAACA640870.140440281718555No Hit
ATTTAGTCTGGTTTCAACAGAAACCAGGGAAAGCCCCAAGGTCCCTTATTT640610.14038330530641707No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC628170.1376572031256646TruSeq Adapter, Index 10 (100% over 51bp)
CTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGT613200.13437667662680092No Hit
TGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCAT609130.1334847766367959No Hit
GTTCATTCTCACCATCAACAGCCTGCAGCCTGAAGATATTGCAGCTTATTA595160.13042339018461652No Hit
CTGGGACAGAGTTCATTCTCACCATCAACAGCCTGCAGCCTGAAGATATTG594180.13020863293886592No Hit
CTGACTCGCCCGACAAGTGATGTTGACTCTGTCTCCTACAGATGCAGACAG588520.12896830027463288No Hit
GTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGC558230.12233054826056601No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATG557420.12215304482275176No Hit
GTGAGAATGAACTCTGTCCCAGATCCGCTGCCGCTGAACTTTGATGGGACC552590.12109459839726668No Hit
CCCCACTCTGCAAACTGGATGTACCATAAATAAGGGACCTTGGGGCTTTCC512060.1122128523051528No Hit
CTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCT503260.1102844198943311No Hit
TGGACCTCCTGCACAAGGACATGAAACACCTGTGGGTCTTCCTCCTCCTGG486240.10655465629976464No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCC481550.10552688948081539No Hit
CCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGG480200.10523105041779159No Hit
GAATTATTTAGTCTGGTTTCAACAGAAACCAGGGAAAGCCCCAAGGTCCCT479520.1050820351860463No Hit
CCCTGACTCGCCCGACAAGTGATGTTGACTCTGTCTCCTACAGATGCAGAC478010.10475113371555302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)