FastQCFastQC Report
Thu 9 May 2024
EGAF00000718623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000718623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45632919
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAA1176320.2577788197156531No Hit
CTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTT1050120.23012334582409683No Hit
CCTGACTCGCCCGACAAGTGATGTTGACTCTGTCTCCTACAGATGCAGAC970020.21257022808468598No Hit
CCCAGATCCGCTGCCGCTGAACTTTGATGGGACCCCACTCTGCAAACTGG905670.19846856608055252No Hit
CTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCT895160.1961654041899007No Hit
CCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC801630.17566923562351994No Hit
GGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTG747010.16369980627362452No Hit
TGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTC726870.15928632573340312No Hit
CGACAAGTGATGTTGACTCTGTCTCCTACAGATGCAGACAGTGAAGATGG697350.15281731155528314No Hit
TGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGT665810.14590563448286092No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC656980.14397062787063875No Hit
CCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGAT643950.14111523306234258No Hit
ATTTAGTCTGGTTTCAACAGAAACCAGGGAAAGCCCCAAGGTCCCTTATT641180.1405082151330271No Hit
CAAAGTTCAGCGGCAGCGGATCTGGGACAGAGTTCATTCTCACCATCAAC641090.14048849252882553No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC628990.13783689796394571TruSeq Adapter, Index 10 (100% over 50bp)
CTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTG613870.1345235004580794No Hit
TGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCA609700.13360968646340593No Hit
GTTCATTCTCACCATCAACAGCCTGCAGCCTGAAGATATTGCAGCTTATT595580.1305154290042239No Hit
CTGGGACAGAGTTCATTCTCACCATCAACAGCCTGCAGCCTGAAGATATT594490.13027656635333804No Hit
CTGACTCGCCCGACAAGTGATGTTGACTCTGTCTCCTACAGATGCAGACA588880.12904719069143922No Hit
GTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAG558880.12247298929091079No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT557610.12219468143162175No Hit
GTGAGAATGAACTCTGTCCCAGATCCGCTGCCGCTGAACTTTGATGGGAC553290.12124799642994567No Hit
CCCCACTCTGCAAACTGGATGTACCATAAATAAGGGACCTTGGGGCTTTC512540.11231803952756123No Hit
CTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGC503510.11033920490600219No Hit
TGGACCTCCTGCACAAGGACATGAAACACCTGTGGGTCTTCCTCCTCCTG486660.10664669511937204No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC481870.10559701429575434No Hit
CCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGG480670.1053340462397332No Hit
GAATTATTTAGTCTGGTTTCAACAGAAACCAGGGAAAGCCCCAAGGTCCC479650.10511052339211525No Hit
CCCTGACTCGCCCGACAAGTGATGTTGACTCTGTCTCCTACAGATGCAGA478590.10487823494262989No Hit

[OK]Adapter Content

Adapter graph