FastQCFastQC Report
Tue 27 Sep 2022
EGAF00000718627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000718627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41374077
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT857620.20728438244072492TruSeq Adapter, Index 22 (97% over 40bp)
CCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAA716220.17310839345129075No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG688500.16640854610484723TruSeq Adapter, Index 22 (97% over 40bp)
GAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTAC608440.14705826549314926No Hit
GTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCC516130.12474719375612899No Hit
CGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCT493610.11930417203023043No Hit
CCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAA459960.1111710600818962No Hit
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAA457890.11067074680602541No Hit
CGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAG414920.10028501663009907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)