FastQCFastQC Report
Sat 11 May 2024
EGAF00000718627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000718627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41374077
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTA858550.20750916086901466TruSeq Adapter, Index 22 (97% over 40bp)
CCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCA716660.17321474023456765No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT689230.166584985086193TruSeq Adapter, Index 22 (97% over 40bp)
GAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTA609110.14722020264041177No Hit
GTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCC516740.1248946290693083No Hit
CGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCC493780.11934526056013287No Hit
CCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCA460390.11127498989282587No Hit
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAA458920.11091969495778721No Hit
CGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAA415190.10035027488347353No Hit

[OK]Adapter Content

Adapter graph