FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000727186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000727186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11951555
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG9153847.659120507749828TruSeq Adapter, Index 2 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC4492513.7589334609596827TruSeq Adapter, Index 2 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATATCGTATG311130.2603259575845988TruSeq Adapter, Index 2 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATATCGTATGC126230.1056180555584608TruSeq Adapter, Index 2 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)