FastQCFastQC Report
Sat 13 May 2023
EGAF00000727192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000727192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42477658
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG4815671.133694800217093TruSeq Adapter, Index 1 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC4587151.079897107321689TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATG519510.12230194046950517TruSeq Adapter, Index 1 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC438070.10312950869372317TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)