FastQCFastQC Report
Sat 1 Apr 2023
EGAF00000727220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000727220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23001468
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTA7192513.126978678056548TruSeq Adapter, Index 22 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT1386950.6029832530688911TruSeq Adapter, Index 22 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATATCGTA349540.1519642137623564TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)