FastQCFastQC Report
Fri 31 Mar 2023
EGAF00000727444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000727444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19843857
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA4774992.406281198256972TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT2543281.2816460025891137TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTA255620.12881568336236246TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)