FastQCFastQC Report
Wed 1 May 2024
EGAF00000727606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000727606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26200700
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA16335406.234718919723519TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT3406411.3001217524722621TruSeq Adapter, Index 18 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATATCGTA421320.1608048639921834TruSeq Adapter, Index 18 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)