FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000727614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000727614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23715111
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTA10888854.591523944374538TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT2613851.1021875461599147TruSeq Adapter, Index 20 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATATCGTA278120.11727543674579469TruSeq Adapter, Index 20 (97% over 43bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)