FastQCFastQC Report
Sat 1 Apr 2023
EGAF00000727640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000727640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21424102
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT15600257.281635421638676TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA12321575.751265560628865TruSeq Adapter, Index 15 (97% over 40bp)
CACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTG1021530.47681345057076374TruSeq Adapter, Index 15 (96% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTAT890740.41576538423874193TruSeq Adapter, Index 15 (97% over 40bp)
AGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCT839230.39172236950701594TruSeq Adapter, Index 15 (96% over 33bp)
GCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCT703680.32845250643410867TruSeq Adapter, Index 15 (96% over 30bp)
ACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGC685300.3198733837245547TruSeq Adapter, Index 15 (96% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTA663050.30948788425297824TruSeq Adapter, Index 15 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT657760.3070187025808596TruSeq Adapter, Index 15 (97% over 40bp)
AAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTC479350.2237433335595583TruSeq Adapter, Index 15 (97% over 34bp)
AGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTC436810.20388719209794653TruSeq Adapter, Index 15 (96% over 31bp)
CACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCT427850.19970498646804424TruSeq Adapter, Index 15 (96% over 27bp)
GAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGT312340.14578907437987368TruSeq Adapter, Index 15 (97% over 35bp)
CGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTG303160.14150418066530862RNA PCR Primer, Index 40 (100% over 29bp)
GAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTT227010.10596010045135146TruSeq Adapter, Index 15 (96% over 32bp)
ACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTT224640.10485386972112064RNA PCR Primer, Index 40 (96% over 29bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)