FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000727648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000727648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25194793
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA628784224.95691073945319TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT9486753.7653613585950083TruSeq Adapter, Index 27 (97% over 44bp)
AGATCGGAAGAGCACATGTCTGAACTCCAGTCACATTCCTTTATCTCGTA1490060.5914158532677763TruSeq Adapter, Index 27 (97% over 41bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA1184590.47017254716083595TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATATCGTA1160970.4607975941695572TruSeq Adapter, Index 27 (97% over 43bp)
AGATCGGAAGAGCACACGTTTGAACTCCAGTCACATTCCTTTATCTCGTA974520.3867942078349284TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGTACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA862630.34238423788597905TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCTAGTCACATTCCTTTATCTCGTA857130.34020124713864486TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAATTCCAGTCACATTCCTTTATCTCGTA741930.2944775136672089TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTTACATTCCTTTATCTCGTA664510.2637489420929158TruSeq Adapter, Index 27 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTTCAGTCACATTCCTTTATCTCGTA444590.17646106479223703TruSeq Adapter, Index 27 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)