FastQCFastQC Report
Sat 1 Apr 2023
EGAF00000727654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000727654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21362253
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG10394664.865900614509154TruSeq Adapter, Index 2 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC9356704.380015534878273TruSeq Adapter, Index 2 (100% over 50bp)
CGGTGGTCGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT305810.14315437608570594TruSeq Adapter, Index 2 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATATCGTATG304450.14251773911674953TruSeq Adapter, Index 2 (97% over 49bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)