FastQCFastQC Report
Sat 1 Apr 2023
EGAF00000727666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000727666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24864913
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG12026814.83685987560061TruSeq Adapter, Index 8 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC4148271.6683227486056356TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATG680120.2735259922284868TruSeq Adapter, Index 8 (97% over 49bp)
CACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCT568580.22866760080761192TruSeq Adapter, Index 8 (100% over 50bp)
ACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTT479720.19293049607694185TruSeq Adapter, Index 8 (100% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTC347870.1399039682946005TruSeq Adapter, Index 8 (100% over 50bp)
CACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTG249840.10047893592066862TruSeq Adapter, Index 8 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)