FastQCFastQC Report
Wed 14 Sep 2022
EGAF00000727672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000727672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29246548
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT24384068.33741472668843TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA16975925.804418353919923TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTAT1401510.4792052723623998TruSeq Adapter, Index 15 (97% over 40bp)
CACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTG1375900.47044868337965906TruSeq Adapter, Index 15 (96% over 29bp)
AGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCT1118590.38246906951206683TruSeq Adapter, Index 15 (96% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTA1049440.3588252534965836TruSeq Adapter, Index 15 (97% over 40bp)
GCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCT899380.30751663409986024TruSeq Adapter, Index 15 (96% over 30bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT862660.2949613062027013TruSeq Adapter, Index 15 (97% over 40bp)
ACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGC843960.28856738921803693TruSeq Adapter, Index 15 (96% over 28bp)
AAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTC627580.21458258937088917TruSeq Adapter, Index 15 (97% over 34bp)
AGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTC600010.20515583582718888TruSeq Adapter, Index 15 (96% over 31bp)
CACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCT582740.1992508654354695TruSeq Adapter, Index 15 (96% over 27bp)
GAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGT404320.1382453751464959TruSeq Adapter, Index 15 (97% over 35bp)
CGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTG389600.13321230252541258RNA PCR Primer, Index 40 (100% over 29bp)
GAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTT313990.10735967882431799TruSeq Adapter, Index 15 (96% over 32bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)