FastQCFastQC Report
Tue 27 Sep 2022
EGAF00000727694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000727694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20313164
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA9191604.524947467563399TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT2234401.0999763503115516TruSeq Adapter, Index 27 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATATCGTA208390.1025886464560617TruSeq Adapter, Index 27 (97% over 43bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)