FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000727718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000727718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19358676
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC243935512.600835924936188TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG7178183.7079911870005984TruSeq Adapter, Index 9 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC929030.47990368762822416TruSeq Adapter, Index 9 (98% over 50bp)
CACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCT436790.2256300999097252TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATG327530.16919028966650404TruSeq Adapter, Index 9 (97% over 49bp)
AGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTG320190.1653987080521416TruSeq Adapter, Index 9 (100% over 50bp)
AAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT299400.15465933723979883TruSeq Adapter, Index 9 (100% over 50bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG250950.12963179919949072Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT239820.12388243906763045TruSeq Adapter, Index 9 (100% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTC228600.11808658815303279TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAACTCGTATGC228600.11808658815303279TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATTTCGTATGC223900.11565873616563448TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)