FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000727802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000727802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20092580
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA2392561.1907679352278302TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1091650.5433100179270158TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTA220370.10967730376089083TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph