FastQCFastQC Report
Wed 14 Sep 2022
EGAF00000728048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000728048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23268286
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG17064687.333879255223183TruSeq Adapter, Index 6 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2827491.2151690072917274TruSeq Adapter, Index 6 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATATCGTATG236960.10183818438539048TruSeq Adapter, Index 6 (97% over 49bp)

[WARN]Adapter Content

Adapter graph