FastQCFastQC Report
Thu 5 Jan 2023
EGAF00000728136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000728136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22337369
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG7926273.54843491191823TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3737021.6729902254826876TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATG473450.21195423686648146TruSeq Adapter, Index 4 (97% over 49bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)