FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000728140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000728140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21875706
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTA8350713.8173442265131925TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT3383041.5464826598053567TruSeq Adapter, Index 7 (97% over 36bp)
CACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTG298640.1365167368769721TruSeq Adapter, Index 7 (96% over 25bp)
ACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGC221900.10143672620211662TruSeq Adapter, Index 7 (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)