FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000728146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000728146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18954699
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC4773832.518546984048652TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG2857671.5076314321847053TruSeq Adapter, Index 9 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC298420.1574385327880965TruSeq Adapter, Index 9 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATG194510.10261835336978972TruSeq Adapter, Index 9 (97% over 49bp)

[OK]Adapter Content

Adapter graph