FastQCFastQC Report
Wed 14 Sep 2022
EGAF00000728166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000728166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23395157
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG11563434.942659713717672TruSeq Adapter, Index 8 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC3573741.527555467997073TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATG901410.38529769216765675TruSeq Adapter, Index 8 (97% over 49bp)
CACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCT442990.18935115502751274TruSeq Adapter, Index 8 (100% over 50bp)
ACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTT409200.1749079948469677TruSeq Adapter, Index 8 (100% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTC284710.12169612710870033TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATGC241370.10317092550394084TruSeq Adapter, Index 8 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)