FastQCFastQC Report
Wed 10 May 2023
EGAF00000728178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000728178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23134585
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG7757023.3529972549756133TruSeq Adapter, Index 8 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC2115870.9145917249001873TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATG667610.2885766051130807TruSeq Adapter, Index 8 (97% over 49bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)