FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000728232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000728232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17199767
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA5164313.002546487984401TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT2593461.5078460074488218TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTA352520.20495626481451756TruSeq Adapter, Index 13 (97% over 40bp)
AGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTC198280.11528063141785583TruSeq Adapter, Index 13 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTAT188550.10962357804033043TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)