FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000728366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000728366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15159705
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTA12800158.443535016017792TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT4161752.745271098613067TruSeq Adapter, Index 20 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATATCGTA261900.17276061770331283TruSeq Adapter, Index 20 (97% over 43bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)