FastQCFastQC Report
Tue 27 Sep 2022
EGAF00000728506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000728506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22211699
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT4707302.1192885785099107TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA3539611.593579131429793TruSeq Adapter, Index 13 (97% over 40bp)
CACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTG285740.12864391868447345TruSeq Adapter, Index 13 (96% over 29bp)
AGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTC262980.1183970663387794TruSeq Adapter, Index 13 (96% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)