FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000728610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000728610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18798460
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA4457282.37108784442981TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT1464830.7792287240550555TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTA326070.17345569796674834TruSeq Adapter, Index 15 (97% over 40bp)
AGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCT192240.10226369606872053TruSeq Adapter, Index 15 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)