FastQCFastQC Report
Thu 2 May 2024
EGAF00000728658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000728658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36483656
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG5084351.393596628583495TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC4793091.3137636206196002TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATG374050.10252536094518597TruSeq Adapter, Index 4 (97% over 49bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)