FastQCFastQC Report
Wed 14 Sep 2022
EGAF00000728686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000728686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30226040
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTA5393371.7843455510546538TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT1139450.37697627608512396TruSeq Adapter, Index 21 (97% over 40bp)
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA314220.10395672076130383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)