FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000728704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000728704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14702932
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG7010454.768062587788612TruSeq Adapter, Index 1 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC5276733.5888964187551164TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATG450520.30641507421784986TruSeq Adapter, Index 1 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC322980.21967047116860772TruSeq Adapter, Index 1 (98% over 50bp)
CGGTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATC186240.12666861276376712TruSeq Adapter, Index 1 (100% over 42bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)