FastQCFastQC Report
Wed 1 May 2024
EGAF00000728998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000728998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27322625
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTA4304411.5754013386341903TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT1039530.38046490774586994TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)