FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000729038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000729038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17156636
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA2575441.5011334389795297TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1594680.9294829126176016TruSeq Adapter, Index 13 (97% over 40bp)
CACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTG364290.21233183474895662TruSeq Adapter, Index 13 (96% over 29bp)
AGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTC300590.17520334405882365TruSeq Adapter, Index 13 (96% over 31bp)
AGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCT281800.16425131360250342TruSeq Adapter, Index 13 (96% over 33bp)
AAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTC277330.16164590774088813TruSeq Adapter, Index 13 (97% over 34bp)
ACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGC261930.15266978911250434TruSeq Adapter, Index 13 (96% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTA214440.12498953757601433TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)