FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000729112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000729112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17954170
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT6750603.759906472980928TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA5557753.095520427844896TruSeq Adapter, Index 13 (97% over 40bp)
CACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTG440260.24521322901587764TruSeq Adapter, Index 13 (96% over 29bp)
AGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTC360990.2010619260038197TruSeq Adapter, Index 13 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTAT280750.15637035852952266TruSeq Adapter, Index 13 (97% over 40bp)
AAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTC265610.14793777712921288TruSeq Adapter, Index 13 (97% over 34bp)
AGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCT260320.14499138640215614TruSeq Adapter, Index 13 (96% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTA253250.14105358253820702TruSeq Adapter, Index 13 (97% over 40bp)
ACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGC231150.12874446437791334TruSeq Adapter, Index 13 (96% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)