FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000729174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000729174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19445762
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA12974676.672235317906288TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT4636632.384391005094066TruSeq Adapter, Index 14 (97% over 44bp)
CGGTGGTCGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTT654690.33667490119440935TruSeq Adapter, Index 8 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATATCGTA241700.12429443495194481TruSeq Adapter, Index 14 (97% over 43bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)