FastQCFastQC Report
Fri 30 Sep 2022
EGAF00000729175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000729175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19445762
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC12869116.617950996211926Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC365140.18777356217771257Illumina Single End PCR Primer 1 (100% over 38bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG323390.16630358841170637Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)