FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000729176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000729176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22139321
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC20386839.208426039804925TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG11328395.116864243487865TruSeq Adapter, Index 9 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC959600.43343696041987917TruSeq Adapter, Index 9 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATG664390.3000950209809958TruSeq Adapter, Index 9 (97% over 49bp)
CACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCT571590.2581786496523538TruSeq Adapter, Index 9 (100% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT318100.14368100990992452TruSeq Adapter, Index 9 (100% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTC285340.12888380813485653TruSeq Adapter, Index 9 (100% over 50bp)
AAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTT280160.12654407964905517TruSeq Adapter, Index 9 (100% over 50bp)
AGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTG227890.10293450282418327TruSeq Adapter, Index 9 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)