FastQCFastQC Report
Sat 1 Apr 2023
EGAF00000729180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000729180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24296107
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG12930885.322202441732744TruSeq Adapter, Index 7 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC6862732.8246212448768024TruSeq Adapter, Index 7 (100% over 50bp)
CGGTGGTCGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA532730.2192655802841171TruSeq Adapter, Index 7 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATATCGTATG458640.18877098293977715TruSeq Adapter, Index 7 (97% over 49bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)