FastQCFastQC Report
Sat 1 Apr 2023
EGAF00000729200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000729200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25405295
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT18365277.228914287356238TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA14364395.654092975499792TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTAT951790.3746423727809498TruSeq Adapter, Index 15 (97% over 40bp)
CACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTG812090.3196538359424679TruSeq Adapter, Index 15 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTA746020.2936474463295939TruSeq Adapter, Index 15 (97% over 40bp)
AGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCT664840.26169347767857054TruSeq Adapter, Index 15 (96% over 33bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT605660.23839912112809553TruSeq Adapter, Index 15 (97% over 40bp)
GCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCT560290.22054063926437384TruSeq Adapter, Index 15 (96% over 30bp)
ACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGC520300.204799826177968TruSeq Adapter, Index 15 (96% over 28bp)
AAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTC380840.14990575783512847TruSeq Adapter, Index 15 (97% over 34bp)
AGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTC359550.14152561503418873TruSeq Adapter, Index 15 (96% over 31bp)
CACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCT343240.1351056935178277TruSeq Adapter, Index 15 (96% over 27bp)
GAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGT280520.11041792665662808TruSeq Adapter, Index 15 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)