FastQCFastQC Report
Sat 1 Apr 2023
EGAF00000729436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000729436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23069023
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA886030.38407781725303236TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT425120.18428175306773936TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)