FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000729544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000729544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16287532
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA1470120.9026045198253486TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT482570.2962818430686736TruSeq Adapter, Index 15 (97% over 40bp)
AGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCT168210.10327531513061648TruSeq Adapter, Index 15 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)