FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000729546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000729546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15978424
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTA2556921.6002329140846432TruSeq Adapter, Index 25 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT642770.4022737161061692TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)