FastQCFastQC Report
Tue 13 Sep 2022
EGAF00000729708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000729708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17452304
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG4511042.5847819290793925TruSeq Adapter, Index 10 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC4241492.430332407686687TruSeq Adapter, Index 10 (100% over 50bp)
CGGTGGTCGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC269020.1541458365611784TruSeq Adapter, Index 10 (100% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)