FastQCFastQC Report
Sat 13 May 2023
EGAF00000729770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000729770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24630947
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA2981381.2104203707636576TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT1739330.7061563649988772TruSeq Adapter, Index 16 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATATCGTA257410.10450674105222182TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)