FastQCFastQC Report
Sun 2 Apr 2023
EGAF00000729866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000729866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30300223
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA6558782.164597930516881TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT3343851.1035727360818433TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATATCGTA870360.28724541070209286TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATATCGTAT412780.13623002048532779TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)